Purification and characterization of endo-.ALPHA.-N-acetylgalactosaminidase from Alcaligenes sp.

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  • Purification and characterization of endo-α-N-acetyl-galactosaminidase from Alcaligenes sp.

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Abstract

A gram negative bacterium isolated from soil was found to produce an endo-α-N-acetylgalactosaminidase in culture. The microorganism was identified as Alcaligenes sp. from various bacteriological characteristics. The enzyme was purified from the culture fluid by fractionation with ammonium sulfate, and column chromatographies on DEAE-Sephadex A-50 and hydroxylapatite. The molecular weight of the enzyme was estimated to be 160, 000 by gel filtration and SDS-polyacrylamide gel electrophoresis. The optimum pH was found to be 4.5 and the stable pH range was 4.5-6.5. The Km values were 3.7mM and 3.2mM with asialofetuin and asialo κ-casein glycopeptide as substrates, respectively. The enzyme released the disaccharide, Galβ1→3GalNAc, from glycoproteins possessing serine or threonine O-glycosidic linkages. The enzyme production was highly induced by porcine gastric mucin added to the medium.

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Details 詳細情報について

  • CRID
    1390001206464237696
  • NII Article ID
    110006323887
  • NII Book ID
    AA00515312
  • DOI
    10.1271/bbb1961.52.1715
  • COI
    1:CAS:528:DyaL1cXls1KitLo%3D
  • ISSN
    18811280
    00021369
    http://id.crossref.org/issn/00021369
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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