Construction of new ARS1 vectors with increased mitotic stability in Saccharomyces cerevisiae.

• KAMAKURA Takashi

  The Institute of Physical and Chemical Research

• NAKAJIMA Harushi

  Department of Agricultural Chemistry, The University of Tokyo

• YODA Koji

  Department of Agricultural Chemistry, The University of Tokyo

• YAMAGUCHI Isamu

  The Institute of Physical and Chemical Research

• YAMASAKI Makari

  Department of Agricultural Chemistry, The University of Tokyo

• TAMURA Gakuzo

  Department of Agricultural Chemistry, The University of Tokyo

Abstract

Versatile plasmids for use of a host-vector system with the yeast, Saccharomyces cerevisiae, were constructed. They contain the ARS1-TRP1 region of YRp7 as the replication origin and a selectable marker, the STB region of 2 μ circle for the plasmid mitotic stability, and a multiple cloning sequence for cloning foreign DN A. They do not contain the "poison sequence" of pBR322. These plasmids are very easy to handle because of their small size and mitotic stability in yeast cells.

Journal

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 52 (8), 2021-2025, 1988

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

Details

• CRID: 1390001206463527552
• NII Article ID: 110006323942
• NII Book ID: AA00515312
• DOI: 10.1271/bbb1961.52.2021
• ISSN: 18811280
• ISSN: 00021369
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed