Efficient Usage of a Galactose-inducible Expression Vector for the Production of Heterologous Protein in Yeast(Microbiology & Fermentation Industry)

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Author(s)

    • ABE Akio
    • Laboratory of Molecular Genetics, Keio University School of Medicine
    • WADA Tadashi
    • Laboratory of Molecular Genetics, Keio University School of Medicine:(Present office)Department of Microbiology, Faculty of Medicine, The University of Tokyo
    • HANDA Hiroshi
    • Laboratory of Molecular Genetics, Keio University School of Medicine:(Present office)Department of Microbiology, Faculty of Medicine, The University of Tokyo
    • NOGI Yasuhisa
    • Laboratory of Molecular Genetics, Keio University School of Medicine
    • FUKASAWA Toshio
    • Laboratory of Molecular Genetics, Keio University School of Medicine

Abstract

We found that the expression vector previously constructed using the promoter of the GAL7 gene of Saccharomyces cerevisiae lacked the transcription terminator. The addition of the GAL7 terminator resulted in a more than 3-fold increase in the production of the protein encoded by cDNA of early region 1a (E1a) of human adenovirus, without affecting the amount of its mRNA. The positive regulatory gene, GAL4, when amplified with a vector of a high copy number, caused an about 5-fold increase in E1a production. We also found that medium containing a mixture of galactose and glucose can be used for the efficient production of the E1a protein.

Journal

  • Agricultural and biological chemistry

    Agricultural and biological chemistry 52(8), 2035-2041, 1988-08-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

Cited by:  1

  • Spectrophotometric and Molecular Properties of Mutated Rice Phytochrome A : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION

    Ken-ichi TOMIZAWA , Jorg STOCKHAUS , Nam-Hai CHUA , Masaki FURUYA , Frontier Research Program Laboratory of Plant Biological Regulation RIKEN Institute , Frontier Research Program Laboratory of Plant Biological Regulation RIKEN Institute:The Rockefeller University Laboratory of Plant Molecular Biology:(Present)Institut fur Entwicklungs und Molekularbiologie der Pflanzen Heinrich-Heine-Universitat , The Rockegeller University Laboratory of Plant Molecular Biology , Frontier Research Program Laboratory of Plant Biological Regulation RIKEN Institute:Advanced Research Laboratory Hitachi Ltd.

    Plant and cell physiology = Plant and cell physiology 36(3), 511-516, 1995-04

    CiNii Fulltext Link IR References (16) Cited by (1)

Codes

  • NII Article ID (NAID)
    110006323944
  • NII NACSIS-CAT ID (NCID)
    AA00515312
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00021369
  • Data Source
    CJPref  NII-ELS  J-STAGE 
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