Efficient usage of a galactose-inducible expression vector for the production of heterologous protein in yeast.

• ABE Akio

  Laboratory of Molecular Genetics, Keio University School of Medicine

• WADA Tadashi

  Laboratory of Molecular Genetics, Keio University School of Medicine Present address: Department of Microbiology, Faculty of Medicine, The University of Tokyo

• HANDA Hiroshi

  Laboratory of Molecular Genetics, Keio University School of Medicine Present address: Department of Microbiology, Faculty of Medicine, The University of Tokyo

• NOGI Yasuhisa

  Laboratory of Molecular Genetics, Keio University School of Medicine

• FUKASAWA Toshio

  Laboratory of Molecular Genetics, Keio University School of Medicine

Abstract

We found that the expression vector previously constructed using the promoter of the GAL7 gene of Saccharomyces cerevisiae lacked the transcription terminator. The addition of the GAL7 terminator resulted in a more than 3-fold increase in the production of the protein encoded by cDNA of early region la (Ela) of human adenovirus, without affecting the amount of its mRNA. The positive regulatory gene, GAL4, when amplified with a vector of a high copy number, caused an about 5-fold increase in Ela production. We also found that medium containing a mixture of galactose and glucose can be used for the efficient production of the Ela protein.

Journal

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 52 (8), 2035-2041, 1988

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

Details

• CRID: 1390001206463528960
• NII Article ID: 110006323944
• NII Book ID: AA00515312
• DOI: 10.1271/bbb1961.52.2035
• ISSN: 18811280
• ISSN: 00021369
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed