Efficient Usage of a Galactose-inducible Expression Vector for the Production of Heterologous Protein in Yeast(Microbiology & Fermentation Industry)

    • ABE Akio
    • Laboratory of Molecular Genetics, Keio University School of Medicine
    • WADA Tadashi
    • Laboratory of Molecular Genetics, Keio University School of Medicine:(Present office)Department of Microbiology, Faculty of Medicine, The University of Tokyo
    • HANDA Hiroshi
    • Laboratory of Molecular Genetics, Keio University School of Medicine:(Present office)Department of Microbiology, Faculty of Medicine, The University of Tokyo
    • NOGI Yasuhisa
    • Laboratory of Molecular Genetics, Keio University School of Medicine

    • FUKASAWA Toshio
    • Laboratory of Molecular Genetics, Keio University School of Medicine

Abstract

We found that the expression vector previously constructed using the promoter of the GAL7 gene of Saccharomyces cerevisiae lacked the transcription terminator. The addition of the GAL7 terminator resulted in a more than 3-fold increase in the production of the protein encoded by cDNA of early region 1a (E1a) of human adenovirus, without affecting the amount of its mRNA. The positive regulatory gene, GAL4, when amplified with a vector of a high copy number, caused an about 5-fold increase in E1a production. We also found that medium containing a mixture of galactose and glucose can be used for the efficient production of the E1a protein.

Journal

Agricultural and biological chemistry   [List of Volumes]

Agricultural and biological chemistry 52(8), 2035-2041, 1988-08-23  [Table of Contents]

Japan Society for Bioscience, Biotechnology, and Agrochemistry

Cited by:  1

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Codes

  • NII Article ID (NAID) :
    110006323944
  • NII NACSIS-CAT ID (NCID) :
    AA00515312
  • Text Lang :
    ENG
  • Article Type :
    Journal Article
  • ISSN :
    00021369
  • Databases :
    CJPref  NII-ELS  Journal@rchive 

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