Polymerization of several proteins by Ca2+-independent transglutaminase derived from microorganisms.

• NONAKA Masahiko

  Central Research Laboratories, Ajinomoto Co., Inc.

• TANAKA Haruo

  Central Research Laboratories, Ajinomoto Co., Inc.

• OKIYAMA Atsusi

  Central Research Laboratories, Ajinomoto Co., Inc.

• MOTOKI Masao

  Central Research Laboratories, Ajinomoto Co., Inc.

• ANDO Hiroyasu

  Amano Pharmaceutical Co., Ltd.

• UMEDA Koichi

  Amano Pharmaceutical Co., Ltd.

• MATSUURA Akira

  Amano Pharmaceutical Co., Ltd.

抄録

α_s1-Casein and soybean globulins were polymerized and gelatinized by Ca₂₊-independent transglutaminase that was isolated from the culture filtrate of a microorganism thought to belong to Streptoverticillium sp. of actinomycetes. This enzyme polymerized such albumins as bovine serum albumin, human serum albumin and conalbumin in the presence of dithiothreitol. Rabbit myosin was polymerized by the present emzyme but actin was not. An RP-HPLC analysis after enzymic digestion of the polymerized α_s1-casein showed existence of the ε-(γ-Glu)Lys bond. Thus, it was confirmed that the polymerization was formed by a catalytic reaction of the transglutaminase.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 53 (10), 2619-2623, 1989

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681441064832
• NII論文ID: 110006324033
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.53.2619
• COI: 1:CAS:528:DyaL1MXmt1OrtL8%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可