Intergeneric hybridization between Aspergillus niger and Trichoderma viride by protoplast fusion.

• KIRIMURA Kohtaro

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• IMURA Masatoshi

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• LEE Sung Pyo

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• KATO Yutaka

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

• USAMI Shoji

  Department of Applied Chemistry, School of Science and Engineering, Waseda University

抄録

The intergeneric hybridization between Aspergillus niger and Trichoderma viride was studied. The fusion of protoplasts from auxotrophic mutant strains, using a polyethylene glycol solution, resulted in the formation of intergeneric fusants, the fusion frequencies being 10^-5 to 10^-4. The fusant strains were classifiable into two types according to their morphologies. The fusant strains of the first type formed prototrophic conidia and colonies showing an A. niger type morphology on both the minimal and supplemented media. These fusant strains were proved to be haploids by measurement of conidial sizes and DN A contents, and assumed to be recombinants. The fusant strains of the second type formed conidia showing the same nutritional requirements as those of the original auxotrophic mutant strains used for protoplast fusion. These fusant strains formed colonies showing mixed morphologies, between those of A. niger and T. viride, on the minimal medium and grew more slowly than the prototrophic parental strains of A. niger and T. viride. From these results and those of conidia analyses, the fusant strains of the second type were proved to be heterokaryons. Mycelial protoplasts of the fusant strains of the two types regenerated on the hypertonic minimal medium into colonies with morphological features identical to those of the original fusant strains.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 53 (6), 1589-1596, 1989

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681439750656
• NII論文ID: 110006324367
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.53.1589
• ISSN: 18811280
• ISSN: 00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可