Tracing metabolic changes in soybean cotyledons during germination by NMR.

ISHIDA Nobuaki, KOBAYASHI Toshio, MASUDA Ryoichi, KANO Hiromi, YOSHIDA Takashi, OGAWA Hidejiro

doi:10.1271/bbb1961.54.1359

The changes in ¹³C-NMR and ³¹P-NMR spectra and ¹H-NMR images in soybean cotyledons during germination were investigated. Using ¹³C-NMR, fatty acid signals in the form of triglycerides were observed in dry seeds, and those were observed approximately 18 days after the start of imbibition. Sucrose signals appeared at 16 hr and disappeared at 5 days. A-⁺N-(CH₃)₃ signal was observed after 5 days, suggesting the activation of membrane metabolism in the cotyledons.<br> ³¹P-NMR signals appeared 2hr after imbibition before any apparent change in the ¹³C-NMR spectrum. The peaks identified as sugar phosphate, inorganic phosphate in the cytoplasm and in the vacuole, and an unassigned compound, were distinguishable after 5 days. The vacuole-associated inorganic phosphate peak became prominent 18 days after imbibition in ³¹P-NMR.<br> Distribution maps of free water indicated that the stored macro-molecular materials which bound water were consumed heterogeneously within the cotyledon. The relaxation time (T₁) increased suddenly between 18 days and 23 days after imbibition, which indicates the consumption of stored materials.<br> These findings suggest that cotyledons are the source of such compounds and the energy required for plant growth for approximately 18 days from germination until tri-foliolate leaves begin developing.

Tracing metabolic changes in soybean cotyledons during germination by NMR.

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Tracing metabolic changes in soybean cotyledons during germination by NMR.

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