Tracing metabolic changes in soybean cotyledons during germination by NMR.

• ISHIDA Nobuaki

  National Food Research Institute, Tsukuba Science City

• KOBAYASHI Toshio

  National Food Research Institute, Tsukuba Science City

• MASUDA Ryoichi

  National Food Research Institute, Tsukuba Science City

• KANO Hiromi

  National Institute of Agrobiological Resources, Tsukuba Science City

• YOSHIDA Takashi

  National Institute of Vegetables, Ornamental Crops, and Tea

• OGAWA Hidejiro

  JEOL Ltd.

Abstract

The changes in ¹³C-NMR and ³¹P-NMR spectra and ¹H-NMR images in soybean cotyledons during germination were investigated. Using ¹³C-NMR, fatty acid signals in the form of triglycerides were observed in dry seeds, and those were observed approximately 18 days after the start of imbibition. Sucrose signals appeared at 16 hr and disappeared at 5 days. A-⁺N-(CH₃)₃ signal was observed after 5 days, suggesting the activation of membrane metabolism in the cotyledons.<br> ³¹P-NMR signals appeared 2hr after imbibition before any apparent change in the ¹³C-NMR spectrum. The peaks identified as sugar phosphate, inorganic phosphate in the cytoplasm and in the vacuole, and an unassigned compound, were distinguishable after 5 days. The vacuole-associated inorganic phosphate peak became prominent 18 days after imbibition in ³¹P-NMR.<br> Distribution maps of free water indicated that the stored macro-molecular materials which bound water were consumed heterogeneously within the cotyledon. The relaxation time (T₁) increased suddenly between 18 days and 23 days after imbibition, which indicates the consumption of stored materials.<br> These findings suggest that cotyledons are the source of such compounds and the energy required for plant growth for approximately 18 days from germination until tri-foliolate leaves begin developing.

Journal

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 54 (6), 1359-1365, 1990

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

Details

• CRID: 1390001206460951808
• NII Article ID: 110006324959
• NII Book ID: AA00515312
• DOI: 10.1271/bbb1961.54.1359
• COI: 1:CAS:528:DyaK3cXltFamtbc%3D
• ISSN: 18811280
• ISSN: 00021369
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed