Formation of a stable ascorbic acid 2-glucoside by specific transglucosylation with rice seed α-glucosidase
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- MUTO Norio
- Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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- SUGA Sadaharu
- Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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- FUJII Kazuko
- Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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- GOTO Kazuko
- Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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- YAMAMOTO Itaru
- Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
書誌事項
- タイトル別名
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- Formation of a stable ascorbic acid 2-glucoside by specific transglucosylation with rice seed .ALPHA.-glucosidase.
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The enzymatic transglucosylation to synthesize a chemically stable form of I -ascorbic acid (AA) was further investigated by using commercially available enzymes. Among various glycosidases examined, only rice seed α-glucosidase could produce a nonreducing and stable glucoside of AA, which was identified as 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G). The enzyme showed the same regioselective transglucosylase activity as rat intestinal α-glucosidase that had been demonstrated by us to be effective in this reaction, although these two enzymes had different pH optima for maltose hydrolysis. The substrate specificity of rice α-glucosidase for AA-2G formation was considerably different from that of rat α-glucosidase. However, both α-glucosidases had high specificity for the α-1, 4-glucosidic linkage but not α-1, 6, suggesting that they can catalyze a preferential transglucosylation to the 2-position but not to the 6-position of AA. Thus, these results allows us to produce a sufficient amount of AA-2G with rice seed α-glucosidase for its application to medicinal and other uses.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 54 (7), 1697-1703, 1990
公益社団法人 日本農芸化学会
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詳細情報
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- CRID
- 1390001206465192704
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- NII論文ID
- 110006325018
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- NII書誌ID
- AA00515312
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- COI
- 1:CAS:528:DyaK3cXlsVOrtL4%3D
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- ISSN
- 18811280
- 00021369
- http://id.crossref.org/issn/00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 使用不可