Formation of a stable ascorbic acid 2-glucoside by specific transglucosylation with rice seed α-glucosidase

  • MUTO Norio
    Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
  • SUGA Sadaharu
    Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
  • FUJII Kazuko
    Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
  • GOTO Kazuko
    Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
  • YAMAMOTO Itaru
    Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University

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タイトル別名
  • Formation of a stable ascorbic acid 2-glucoside by specific transglucosylation with rice seed .ALPHA.-glucosidase.

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The enzymatic transglucosylation to synthesize a chemically stable form of I -ascorbic acid (AA) was further investigated by using commercially available enzymes. Among various glycosidases examined, only rice seed α-glucosidase could produce a nonreducing and stable glucoside of AA, which was identified as 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G). The enzyme showed the same regioselective transglucosylase activity as rat intestinal α-glucosidase that had been demonstrated by us to be effective in this reaction, although these two enzymes had different pH optima for maltose hydrolysis. The substrate specificity of rice α-glucosidase for AA-2G formation was considerably different from that of rat α-glucosidase. However, both α-glucosidases had high specificity for the α-1, 4-glucosidic linkage but not α-1, 6, suggesting that they can catalyze a preferential transglucosylation to the 2-position but not to the 6-position of AA. Thus, these results allows us to produce a sufficient amount of AA-2G with rice seed α-glucosidase for its application to medicinal and other uses.

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詳細情報

  • CRID
    1390001206465192704
  • NII論文ID
    110006325018
  • NII書誌ID
    AA00515312
  • DOI
    10.1271/bbb1961.54.1697
  • COI
    1:CAS:528:DyaK3cXlsVOrtL4%3D
  • ISSN
    18811280
    00021369
    http://id.crossref.org/issn/00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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