Prothoracicotropic hormone of the silkworm, Bombyx mori: Amino acid sequence and dimeric structure.

• KATAOKA Hiroshi

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• NAGASAWA Hiromichi

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• ISOGAI Akira

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• ISHIZAKI Hironori

  Biological Institute, Faculty of Science, Nagoya University

• SUZUKI Akinori

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

抄録

Prothoracicotropic hormone (PTTH) of the silkworm, Bombyx mori, was purified and its primary structure determined for the most part. From sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified PTTH under non-reducing and reducing conditions, and the amino acid sequence and composition analyses of the carboxamidomethylated PTTH, we concluded that Bombyx PTTH has a dimeric structure consisting of two identical, or nearly identical subunits, held together by disulfide bond(s). There exist subunit variants that differ by deletion of only a few amino acid residues at the N-terminus, and possibly at the C-terminus also, giving rise to a high heterogeneity in the PTTH molecule. The amino acid sequence up to the 104th residue from the N-terminus of the longest subunit, except for the 41st residue, was determined by sequence analysis of fragment peptides produced by lysyl endopeptidase, chymotrypsin and V8 protease digestions, leaving only a short C-terminal sequence undetermined. Bombyx PTTH is expected to contain a carbohydrate chain bound to an asparagine at position 41, deduced from the cDNA sequence.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 55 (1), 73-86, 1991

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206463135744
• NII論文ID: 110006325177
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.55.73
• COI: 1:CAS:528:DyaK3MXhtFOiurc%3D
• ISSN: 18811280
• ISSN: 00021369
• PubMed: 1368675
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可