Microbial Stereoselective Hydrolysis of (.+-.)-cis,trans-Ethyl Chrysanthemate for Preparation of (+)-trans-Acid.
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- MITSUDA Satoshi
- Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co.
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- KOMAKI Ryohei
- Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co.
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- HIROHARA Hideo
- Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co.
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- NABESHIMA Shigeyasu
- Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co.
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Microbial Stereoselective hydrolysis of (±)-cis, trans-ethyl chrysanthemate was examined to prepare (+)-trans-chrysanthemic acid. Through the screening of over 228 strains from culture collections, 31 strains were found to have hydrolytic ability. Stereoselective hydrolysis by Arthrobacter (A.) globiformis IFO-12958 gave the optically pure (+)-trans-aciA. The composition of cultivation media for production of the hydrolytic activity (the responsible esterase) was optimized. The optimum pH and temperature for hydrolysis of ethyl chrysanthemate were pH 10 and 55°C, respectively. Cells of A. globiformis IFO-12958 were treated with a chemical mutagen and the advantageous mutant SC-6-98-28, the hydrolytic activity of which was enhanced 2.5-fold, was isolated.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 55 (11), 2865-2870, 1991
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681436527360
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- NII論文ID
- 110006325293
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- NII書誌ID
- AA00515312
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- COI
- 1:CAS:528:DyaK38Xht1Krur4%3D
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- ISSN
- 18811280
- 00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可