Structural analysis of repetitive DNA sequences in the goat growth hormone gene region.

  • YAMANO Yoshiaki
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
  • ABE Masaki
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
  • MIKAWA Satoshi
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
  • KIOKA Noriyuki
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
  • MANABE Eichi
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
  • SAKAI Hiroshi
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
  • KOMANO Tohru
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University Tohru KOMANO: Laboratory of Biochemistry, Kyoto University
  • UTSUMI Kyozo
    Laboratory of Animal Reproduction, Department of Animal Science, Kyoto University
  • IRITANI Akira
    Laboratory of Animal Reproduction, Department of Animal Science, Kyoto University

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抄録

Two clones that contain goat growth hormone (gGH) genes were isolated from goat genomic library using goat growth hormone cDNA as a probe. One clone CgGH contained gGHl gene, and another clone, EgGH, contained gGH2 and gGH3 genes. The clone EgGH contained 491 bp inserted sequence, just upstream of the gGH3 gene, which was not present in the clone CgGH. From the sequencing data of the flanking regions of these gGH genes, short interspersed repetitive sequences (SINES) were found. Four SINES's existed in the CgGH clone and eight SINES's existed in the EgGH clone. The number of the repetitive DNA sequences in the goat genome was estimated about 102 copies from the analysis of the reassociation rate. As the number of gGH genes (gGHl, gGH2, and gGH3) per genome was different among individual goats, DNA fragments containing gGHl gene, and that containing gGH2 and gGH3 genes, were estimated to be allelic on the goat chromosome.

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詳細情報

  • CRID
    1390001206465035264
  • NII論文ID
    110006325411
  • NII書誌ID
    AA00515312
  • DOI
    10.1271/bbb1961.55.633
  • ISSN
    18811280
    00021369
  • PubMed
    1368627
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • PubMed
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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