The synthesis of new xylosyloligosaccharides by transxylosylation with Aspergillus niger β-xylosidase
-
- KIZAWA Hideki
- Institute of Applied Biochemistry, University of Tsukuba Present address: Intergrated Technology Laboratories, Takeda Chemical Industries, Ltd.
-
- SHINOYAMA Hirofumi
- Institute of Applied Biochemistry, University of Tsukuba Present address: Department of Agricultual Chemistry, Faculty of Horticulture, Chiba University
-
- YASUI Tsuneo
- Institute of Applied Biochemistry, University of Tsukuba
書誌事項
- タイトル別名
-
- The synthesis of new xylosyloligosaccharides by transxylosylation with Aspergillus niger .BETA.-xylosidase.
この論文をさがす
抄録
The synthesis of Xylosyloligosaccharides from β-(1 → 4)-xylobiose in the presence of D-mannose by transxylosylation with β-xylosidase from Aspergillus niger IFO 6662 was studied. At first, the transxylosylation products were separated by charcoal column chromatography in 5% ethanol elution into three peaks, P-l, P-2, and P-3. They were further separated on thin layer chromatography, and their three products, saccharides O-4, O-5, and O-N were purified by preparative paper chromatography. Saccharides O-4 and O-5 were identified as O-β-D-xylopyranosyl-(1 → 4)-D-mannopyranose and O-β-D-xylopyranosyl-(1 → 6)-D-mannopyranose, respectively, by measurement of the degree of polymerization, specific rotation, acid hydrolysis, and methylation analysis. By 1H-NMR spectroscopy in addition to these analyses, saccharide O-N was identified as O-β-D-xylopyranosyl-(1 → 1')-β-D-xylopyranose, a new nonreducing xylobiose.
収録刊行物
-
- Agricultural and Biological Chemistry
-
Agricultural and Biological Chemistry 55 (3), 671-678, 1991
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報
-
- CRID
- 1390001206460959104
-
- NII論文ID
- 110006325417
-
- NII書誌ID
- AA00515312
-
- COI
- 1:CAS:528:DyaK3MXktV2itb4%3D
-
- ISSN
- 18811280
- 00021369
-
- PubMed
- 1368628
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可