Purification and characterization of xylanase from the mid-gut gland of the apple snail (Pomacea canaliculata).
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- SUZUKI Manpei
- National Research Institute of Fisheries Science, Ministry of Agriculture, Forestry and Fisheries
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- YOSHIDA Katsutoshi
- National Research Institute of Fisheries Science, Ministry of Agriculture, Forestry and Fisheries
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- ASHIDA Katsuro
- National Research Institute of Fisheries Science, Ministry of Agriculture, Forestry and Fisheries
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At least fourteen or fifteen kinds of endo-xylanase components (endo-X) were found to participate in the xylan-hydrolysing activity of the mid-gut gland of the apple snail (Pomacea canaliculatd). One of these endo-X was purified to electrophoretic homogeneity from the mid-gut glands of the female snails. Its molecular weight and isoelectric point were estimated to be about 42, 000 by SDS-PAGE and 7.45, respectively. The optimum pH of the enzyme reaction existed between 6.0-6.3, and the activity was stable from pH 4.5-9.0, and was stable for 15min at 40°C and pH 6.0. The activity of this enzyme rose markedly in the presence of some chlorides or sodium bromide, but not in the presence of sodium sulfate or sodium citrate.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 55 (3), 693-700, 1991
公益社団法人 日本農芸化学会
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詳細情報
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- CRID
- 1390282681441797504
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- NII論文ID
- 110006325420
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- NII書誌ID
- AA00515312
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- ISSN
- 18811280
- 00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可