Purification and characterization of aromatic acid reductase from Nocardia asteroides JCM 3016.
-
- KATO Nobuo
- Department of Biotechnology, Faculty of Engineering, Tottori University
-
- JOUNG Eun-Ho
- Department of Biotechnology, Faculty of Engineering, Tottori University Present address: Institute of Biotechnology, Korea University
-
- YANG Han-Chul
- Department of Biotechnology, Faculty of Engineering, Tottori University Present address: Institute of Biotechnology, Korea University
-
- MASUDA Muneto
- Department of Biotechnology, Faculty of Engineering, Tottori University
-
- SHIMAO Masayuki
- Department of Biotechnology, Faculty of Engineering, Tottori University
-
- YANASE Hideshi
- Department of Biotechnology, Faculty of Engineering, Tottori University
この論文をさがす
抄録
Aromatic acid reductase (aryl-aldehyde dehydrogenase, EC 1.2.1.30) was purified to electrophoretic homogeneity from Nocardia asteroides JCM 3016. The enzyme was a monomeric protein with a molecular weight of 152, 000. The enzyme absolutely required ATP, NADPH, and MgCl2 for the reduction of benzoate to benzaldehyde. In the overall reaction, benzaldehyde, AMP, and NADP+ were stoichiometrically formed from benzoate, ATP and NADPH, respectively. The purified enzyme catalyzed the reduction of benzoyl adenosine 5'-monophosphate (bzAMP), which is the intermediate formed from benzoate and ATP. The temperature dependence of the benzoate- and bzAMP-reducing activities agreed exactly, and the same Km for NADPH was obtained by the steady state kinetics of both reactions. These results suggest that the overall reduction of benzoate proceeds via bzAMP, and that the ATP-dependent adenylation of benzoate and the reduction of bzAMP are catalyzed by one enzyme. The enzyme was fairly specific for meta-substituted benzoates.
収録刊行物
-
- Agricultural and Biological Chemistry
-
Agricultural and Biological Chemistry 55 (3), 757-762, 1991
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報
-
- CRID
- 1390282681441795584
-
- NII論文ID
- 110006325429
-
- NII書誌ID
- AA00515312
-
- ISSN
- 18811280
- 00021369
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可