Polymorphism of acid and neutral .ALPHA.-glucosidases in banana pulp:Changes in apparent pIs and affinity to Con A of the enzymes during ripening.

• KONISHI Yôtarô

  Department of Food and Nutrition, Faculty of Science of Living, Osaka City University

• KITAZATO Sachiko

  Department of Food and Nutrition, Faculty of Science of Living, Osaka City University

• ASANO Ryôko

  Department of Food and Nutrition, Faculty of Science of Living, Osaka City University

• NAKATANI Nobuji

  Department of Food and Nutrition, Faculty of Science of Living, Osaka City University

抄録

We observed the presence of acid and neutral α-glucosidases with optimum pHs of 4.5 and 6.5, respectively, and their multiple forms in banana pulp. By chromatofocusing analysis, each α-glucosidase from preclimacteric bananas was found to be of pI > 7.4, which disappeared upon ripening, although another form with pI 5 as a major component appeared instead. It is unknown whether this phenomenon is due to protein de novo synthesis and/or post-translational modifications of the enzyme. A large amount of acid α-glucosidase from preclimacteric bananas was shown to be a glycoprotein that bound to a Con A-Sepharose column. While this type of enzyme decreased during ripening, it remained a possibility that sugar components of the enzyme might be processed during ripening. The neutral α-glucosidase had no affinity to the column through ripening. These results provide preliminary information on the mechanism of appearance of α-glucosidase multiforms as well as on a useful strategy for purification of acid and neutral α-glucosidases from banana pulp at various stages of ripening.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 55 (4), 1089-1094, 1991

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681437171840
• NII論文ID: 110006325492
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.55.1089
• COI: 1:CAS:528:DyaK3MXkt1Kjurw%3D
• ISSN: 18811280
• ISSN: 00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可