Structures and activity of angiotensin-converting enzyme inhibitors in an α-zein hydrolysate

• MIYOSHI Shinsuke

  Research and Development Center, Showa Sangyo Co., Ltd.

• ISHIKAWA Hiromi

  Research and Development Center, Showa Sangyo Co., Ltd.

• KANEKO Toshiyuki

  Research and Development Center, Showa Sangyo Co., Ltd.

• FUKUI Fumio

  Research and Development Center, Showa Sangyo Co., Ltd.

• TANAKA Hideoki

  Fermentation Research Institute

• MARUYAMA Susumu

  Fermentation Research Institute

書誌事項

タイトル別名

• Structures and activity of angiotensin-converting enzyme inhibitors in an .ALPHA.-zein hydrolysate.

抄録

Peptides that inhibit angiotensin-converting enzyme (ACE) were isolated from α-zein hydrolysate prepared with thermolysin. Their chemical structures were identified by Edman degradation and fast-atom bombardment mass spectrometry. Most of them were found to be tripeptides such as Leu-Arg-Pro, Leu-Ser-Pro, and Leu-Gin-Pro, having IC₅₀ values of 0.27, 1.7, and 1.9μM, respectively. These peptides were synthesized by a solid phase procedure and had similar ACE inhibitory activities as the isolated inhibitors. The hypotensive activity of Leu-Arg-Pro on spontaneously hypertensive rats was also investigated, with the result that the blood pressure decreased by 15mmHg after a 30mg/kg intravenous injection.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 55 (5), 1313-1318, 1991

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681439483136
• NII論文ID: 110006325540
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.55.1313
• COI: 1:CAS:528:DyaK3MXmsVOntro%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• PubMed: 1368684
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可