Synthesis of 2-O-α-d-glucopyranosyl l-ascorbic acid by cyclodextrin glucanotransferase from Bacillus stearothermophilus

• AGA Hajime

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• YONEYAMA Masaru

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• SAKAI Shuzo

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• YAMAMOTO Itaru

  Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University

書誌事項

タイトル別名

• Synthesis of 2-O-.ALPHA.-D-Glucopyranosyl L-Ascorbic Acid by Cyclomaltodextrin Glucanotransferase from Bacillus stearothermophilus.
• Synthesis of O-α-D-glucopyranosyl L-ascorbic acid by Bacillus stearothermophilus
• Synthesis of 2-O-α-d-glucopyranosyl l-ascorbic acid by cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus

抄録

Cyclomaltodextrin glucanotransferase (CGTase) [EC 2.4.1.19] was found to catalyze the transglycosylation from α-cyclodextrin (α-CD) to L-ascorbic acid (AA). A main product formed by this reaction was identified as 2-O-α-D-glucopyranosyl L-ascorbic acid (AA-2G) with the enzymatic hydrolysis and ultraviolet absorption spectra using standard AA-2G. A series of maltooligosaccharide substituted 2-O-derivatives of AA were also detected by HPLC. These were thoroughly hydrolized to AA-2G and glucose by treatment with glucoamylase [EC 3.2.1.3].<br> A large amount of AA-2G was prepared with 500 g of AA and 1000 g of α-CD. Two hundred and fifty grams of purified AA-2G, the content of which was 97%, was obtained through enzyme reactions and purification with HPLC using an ion-exchange resin.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 55 (7), 1751-1756, 1991

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681438897792
• NII論文ID: 110006325628
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.55.1751
• COI: 1:CAS:528:DyaK3MXlsFOjs7g%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可