Protective Mechanism of Adenosine to the Rat Arterial Endothelial Dysfunction Induced by Hydrogen Peroxide

• Lu Jun

  Department of Pharmacology, School of Medicine, Xi'an Jiaotong University

• Zhu Shu-Ming

  Department of Pharmacology, School of Medicine, Xi'an Jiaotong University

• Zang Wei-Jin

  Department of Pharmacology, School of Medicine, Xi'an Jiaotong University Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, School of Medicine, Xi'an Jiaotong University

• Xu Xiao-Li

  Department of Pharmacology, School of Medicine, Xi'an Jiaotong University

• Luo Hong-Li

  Department of Pharmacology, School of Medicine, Xi'an Jiaotong University

• Yu Xiao-Jiang

  Department of Pharmacology, School of Medicine, Xi'an Jiaotong University

• Wang Sheng-Peng

  Department of Pharmacology, School of Medicine, Xi'an Jiaotong University

• Kong Shan-Shan

  Department of Pharmacology, School of Medicine, Xi'an Jiaotong University

• Wu Jie

  Department of Pharmacology, School of Medicine, Xi'an Jiaotong University Department of Cardiovascular and Respiratory Medicine, Shiga University of Medical Science

• Horie Minoru

  Department of Cardiovascular and Respiratory Medicine, Shiga University of Medical Science

• Sun Lei

  Department of Pharmacology, School of Medicine, Xi'an Jiaotong University

Abstract

This study was designed to examine the in vitro effects of adenosine (Ado) on hydrogen peroxide-induced endothelial dysfunction in rats. Endothelial dysfunction was induced by exposing isolated rat mesenteric arteries to hydrogen peroxide (0.5 mM) for 12 h using an organ culture system. The protective effects of adenosine were tested by exposing isolated mesenteric arteries to adenosine (3×10⁻⁷ mol/l, 10⁻⁶ mol/l, 3×10⁻⁶ mol/l)+hydrogen peroxide (0.5 mM) for 12 h. This exposure to hydrogen peroxide induced a significant concentration-dependent inhibition of endothelium-dependent relaxation (EDR). Coculture of segments of mesenteric artery with adenosine (3×10⁻⁷, 10⁻⁶, and 3×10⁻⁶ mol/l) attenuated the hydrogen peroxide-induced impairment of vasorelaxation. This impairment was accompanied by a reduction in nitrite/nitrate, nitric oxide (NO) synthase (NOS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and an increasing in malondislehyde (MDA) and lactate dehydrogenase (LDH) activities in the aorta. These results indicate that adenosine can be used to attenuate hydrogen peroxide-induced endothelial dysfunction, an effect that may be related to antioxidation, thus enhancing NO production by preventing the decrease in NOS.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 30 (7), 1206-1211, 2007

  The Pharmaceutical Society of Japan

Keywords

• hydrogen peroxide
• adenosine
• endothelium-dependent relaxation (EDR)
• nitric oxide (NO)
• nitric oxide synthase (NOS)

Details

• CRID: 1390282679603017600
• NII Article ID: 110006342733
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.30.1206
• COI: 1:STN:280:DC%2BD2szos1arsg%3D%3D
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 8784653
• Web Site: http://id.ndl.go.jp/bib/8784653
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8784653
• Web Site: http://www.jstage.jst.go.jp/article/bpb/30/7/30_7_1206/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008017233
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed