LAMP法による幼苗検定法を利用した黄化葉巻病抵抗性トマト系統の育成(育種・遺伝資源)  [in Japanese] Development of Tomato Strains Resistant to Tomato Yellow Leaf Curl Virus by the Seedling Test Using the LAMP Method(Breeding & Germplasm Resources)  [in Japanese]

トマト黄化葉巻ウイルス(TYLCV)に抵抗性を持ち,わが国の消費嗜好に適した生食用トマト系統(Lycopersicon esculentum)を育成する目的で,オランダから導入した抵抗性品種'Athyla'と国内品種'桃太郎ファイト'を交雑して,選抜・固定を行った.LAMP法を活用した幼苗検定法で選抜を行い,感染はするが,植物体内でのウイルス増殖が抑制され,病徴を発現しないTYLCV抵抗性系統を得た.得られた系統は,わが国の一般的な生食用大玉品種と同様に,果実は大玉で果皮色が無色,糖度が高いなどの特性を有する.LAMP法を活用した幼苗検定法によるTYLCV抵抗性とトマト黄化葉巻病常発地は場での抵抗性は相関が高く,幼苗検定法による選抜は有効な育種手法であると考えられた.

To develop tomato strains (Lycopersicon esculentum Mill.) with Tomato yellow leaf curl virus (TYLCV) resistance and suitable characteristics for eating raw in Japan, the resistant variety 'Athyla' introduced from Holland and 'Momotaro Fight' were crossed, and progeny strains were selected and fixed. TYLCV resistant strains which were infected with the virus, but show characteristics of resistance such as suppression of viral growth in the plant and prevention of disease symptoms, were obtained by seedling test using the loop-mediated isothermal amplification (LAMP) method. Those strains show the same fruit characteristics as the typical Japanese cultivar such as big fruit size, clear fruit skin color, high fruit concentration of soluble solids and so on. It is considered that the seedling test using the LAMP method is suitable for breeding TYLCV resistant tomato, because high correlation was shown between resistance by this method and field test resistance in the TYLCV epidemic district.