キウイフルーツの溶液受粉における効率的花粉懸濁液調整法(栽培管理・作型)  [in Japanese] Labor-saving Preparation Method of Pollen Suspension for Artificial Pollination of Kiwifruit(Crop Production & Cropping Type)  [in Japanese]

キウイフルーツの溶液受粉において,花粉懸濁液を効率的に調整する新たな方法を開発した.生葯を紙の上で乾燥させ開葯した粗花粉を直接液体増量剤(0.1%寒天,5%ショ糖)に懸濁し,約0.12mm目のメッシュで濾過し,花粉懸濁液とした.液体増量剤容量に対する生葯の重量割合が8%以上から得られた花粉懸濁液で,受粉に十分な花粉濃度の懸濁液が得られた.また,400nmの吸光度値から,調整した懸濁液の花粉濃度を推定できた.この花粉懸濁液によって受粉した'ヘイワード'果実の結実率及び果実肥大,果実品質は,純花粉を0.5%(w/v)で希釈した懸濁液で受粉した果実と同等であった.以上のことより,本技術は花粉精製のステップを省くことかでき,キウイフルーツの人工受粉において効率性,実用性の面で有効であると考えられた.

A new labor-saving method for preparing pollen suspension was developed for spray pollination of kiwifruit. Fresh anthers were dried on paper for anthesis. The dried anthers were directly suspended in the liquid extender (0.1% agar, 5% sucrose) and filtered through a 0.12 mm mesh. Sufficient pollen density in the suspension was obtained using more than 8% anthers (w/v) on a fresh weight basis. The pollen density of the preparation was determined by measuring the suspension at 400 nm using a spectrophotometer. There were no significant differences in fruit set, fruit weight, number of seeds or fruit quality in 'Hayward' when efficacy was compared between the newly developed preparation and the suspension prepared from pollen grains (0.5% [w/v]) that we formerly developed. The new preparation will eliminate the step previously performed to collect pollen grains from dried anthers and is thus a more labor-saving method for the artificial pollination of kiwifruit.