日本国内で1993年から2004年に検出された伝染性ファブリキウス嚢病ウイルスVP2超可変領域の塩基配列解析

DOI 日本農学文献記事索引 Web Site Web Site 被引用文献1件 参考文献53件
  • YAMAGUCHI Tsuyoshi
    Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University
  • KASANGA Christopher J.
    Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University
  • TERASAKI Kaori
    Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University
  • MAW Min Thein
    Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University
  • OHYA Kenji
    Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University
  • FUKUSHI Hideto
    Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University

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タイトル別名
  • Nucleotide Sequence Analysis of VP2 Hypervariable Domain of Infectious Bursal Disease Virus Detected in Japan from 1993 to 2004
  • Nucleotide sequence analysis of VP2 hypervariable domain of infectious bursal disease virus detected in Japan from l993 to 2004

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抄録

Bursae of Fabricius were collected from 20 chickens diagnosed with infectious bursal disease virus (IBDV) infection from 15 prefectures in 1993 to 2004. Here we report the nucleotide sequence analysis of VP2 hypervariable domain of IBDV genome detected by reverse transcription-polymerase chain reaction from these samples. Ten sequences derived from 10 prefectures in 1996 to 2003 were of the classical type and other 10 sequences derived from 6 prefectures in 1993 to 2004 were of the highly virulent type. Of the classical type sequences, 9 sequences were closely related to the sequence of classical attenuated vaccines used in Japan. Furthermore, two were identical to the sequence of B-Chi5 which represents Vaccine B passaged 5 times in chickens and was reported to be reverted the virulence during the passages. The 10 highly virulent type sequences were classified into four sequences, none of which had been previously detected in Japan. However, the deduced amino acid sequences were identical to each other and to the sequences of highly virulent IBDVs previously detected in Japan. The most common nucleotide sequences, which accounted for 6 of the sequences, were identical to 34 highly virulent type sequences detected in various countries in BLAST search. This is the first report of detection of the sequence in Japan which is identical to highly virulent strains detected in other countries. These findings show the prevalence of classical IBDVs closely related to the attenuated vaccines and highly virulent IBDVs derived from other countries throughout Japan since 1993.<br>

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