紫外・可視微分分光法及び核磁気共鳴法による分光分析学的薬学研究

• 北村 桂介

  京都薬科大学

書誌事項

タイトル別名

• Derivative Spectrophotometric and NMR Spectroscopic Study in Pharmaceutical Science
• シガイ カシ ビブン ブンコウホウ オヨビ カク ジキ キョウメイホウ ニ ヨル ブンコウ ブンセキガクテキ ヤクガク ケンキュウ

抄録

  This review starts with an introduction of derivative spectrophotometry followed by a description on the construction of a personal computer-assisted derivative spectrophotometric (DS) system. An acquisition system for inputting digitalized absorption spectra into personal computers and a BASIC program for calculating derivative spectra were developed. Then, applications of the system to drug analyses that are difficult with traditional absorption methods are described. Following this, studies on the interactions of drugs with biological macromolecules by the DS and NMR methods were discussed. An ¹H NMR study elucidated that the small unilamellar vesicle (SUV) has a single membrane made of a phosphatidylcholine bilayer, and that chlorpromazine interacts with both the outer and inner layers. ¹³C NMR revealed a reduction of the dissociation constants of phenothiazine drugs due to their interaction with SUV. The partition coefficients of phenothiazine, benzodiazepine and steroid drugs in an SUV-water system and the effects of cholesterol or amino lipids content on these partition coefficients were examined by the DS method. The binding constants of phenothiazine drugs to bovine serum albumin (BSA) and the influence of Na⁺, K⁺, Cl^-, Br^-, and I^- on these binding constants were determined by DS. It was found that I^-, Br^-, Cl^- reduce the binding constants in this order, and that Na⁺ and K⁺ have no effect. A ¹⁹F NMR study revealed that triflupromazine binds to BSA and human serum albumin in two regions including Site II with different populations, and that a nonsteroidal anti-inflammatory drug, niflumic acid, binds Sites Ia and Ib.<br>

収録刊行物

• 薬学雑誌

  薬学雑誌 127 (10), 1621-1642, 2007-10-01

  公益社団法人 日本薬学会

キーワード

• derivative spectrophotometry
• NMR
• interaction
• liposome
• serum albumin

詳細情報

• CRID: 1390001206127088896
• NII論文ID: 110006402948
• NII書誌ID: AN00284903
• DOI: 10.1248/yakushi.127.1621
• ISSN: 13475231
• ISSN: 00316903
• NDL書誌ID: 8964543
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8964543
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/127/10/127_10_1621/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008081333
• 本文言語コード: ja
• データソース種別:
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  • NDL
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• 抄録ライセンスフラグ: 使用不可