Does human immunodeficiency virus type-1 integrate in chromosomes randomly?

• Mostafa Saadat

• Hirokazu Takahashi

• Mirza Khalil Bahmani

Bibliographic Information

Other Title

• Does human immunodeficiency virus type-

Abstract

During the life cycle of retroviruses, including human immunodeficiency virus type 1 (HIV-1), integration of a double stranded DNA copy of viral genome into the host cellular DNA is essentially required. The integration sites of HIV-1 genome within the persistently infected human leukemia cell lines were investigated by fluorescence in situ hybridization (FISH) process using a NarI/XhoI fragment of pNL432 clone of HIV-1 as a probe. In L-2, 15L, 26L and 5H cell lines, each HIV-1 integration site was assigned to 8p11.2, Xp11.3-p11.4, 20q13.1-q13.3, and 21q22.1-q22.3, respectively. In the MOLT-4/LAV-1 cell line HIV-1 was integrated on two chromosomes 1q21-q23 and 8q11-q12. These integration sites appeared to be correspondent to loci of oncogenes and genes related to DNA synthesis and cell cycle.

Journal

• Chromosome Science

  Chromosome Science 2 (1), 5-8, 1998

  Tokyo : The Society of Chromosome Research

Keywords

• HIV-1
• Integration sites
• Chromosomes
• FISH
• Oncogenes

Details

• CRID: 1520854805689779968
• NII Article ID: 110006404850
• NII Article ID: 10004285708
• NII Book ID: AA11163057
• ISSN: 13441051
• NDL BIB ID: 4605346
• Web Site: http://id.ndl.go.jp/bib/4605346
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4605346
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10488668
• Web Site: https://search.jamas.or.jp/link/ui/1999066433
• Text Lang: en
• NDL Source Classification:
  • ZR1(科学技術--生物学)
• Data Source:
  • NDL
  • NDL-Digital
  • CiNii Articles