30.イネのジャスモン酸応答性転写因子RERJ1に発現制御されるchitinase遺伝子のプロモーター解析(口頭発表)

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書誌事項

タイトル別名
  • 30. Promoter analysis of the chitinase gene that is regulated by RERJ1, a jasmonic acid-responsive transcription factor in rice

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Jasmonic acid (JA) is a plant hormone that acts as a secondary signaling molecule in plant defense responses. RERJ1 that encodes a bHLH transcription factor was isolated as a JA-responsive gene from rice suspension cells. Previously, we reported that RERJ1 localized nuclear and functions as a transcriptional activator, and that a rice chitinase gene was screened as a RERJ1-taraget gene by the microarray analysis using RERJ1 overexpressor. The chitinase mRNA levels peaked at 4-6 h after JA treatment in rice cells. Besides, the expression of chitinase gene was induced by in a RERJ1-dependent manner in the transgenic rice in which RERJ1 expression could be controlled by an estrogen treatment. Here, we identified JA-responsive cis element in the promoter region of the chitinase by reporter-gene assay. We conducted the deletion analysis of the chitinase promoter regions located from -2415 to -265 bp upstream of the translation start site. Deletions from -2415 to -515 bp did not have a significant effect on responsiveness to JA, but a further deletion to -265 bp rendered the promoter incapable of responding to JA. This result suggests that the region from -515 to -265 bp contains JA-responsive cis element. Indeed, the region contains three E-box elements, which are putative binding sites for bHLH transcription factors. The mutation analysis showed that an E-box at -448 to -443 bp is essential for responding to JA. Direct binding of RERJ1 to the E-box is now being examined.

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詳細情報 詳細情報について

  • CRID
    1390001204423818624
  • NII論文ID
    110006474723
  • NII書誌ID
    AA11550064
  • DOI
    10.18978/jscrpanb.42.0_43
  • ISSN
    21896313
    09191887
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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