30.イネのジャスモン酸応答性転写因子RERJ1に発現制御されるchitinase遺伝子のプロモーター解析(口頭発表)
書誌事項
- タイトル別名
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- 30. Promoter analysis of the chitinase gene that is regulated by RERJ1, a jasmonic acid-responsive transcription factor in rice
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Jasmonic acid (JA) is a plant hormone that acts as a secondary signaling molecule in plant defense responses. RERJ1 that encodes a bHLH transcription factor was isolated as a JA-responsive gene from rice suspension cells. Previously, we reported that RERJ1 localized nuclear and functions as a transcriptional activator, and that a rice chitinase gene was screened as a RERJ1-taraget gene by the microarray analysis using RERJ1 overexpressor. The chitinase mRNA levels peaked at 4-6 h after JA treatment in rice cells. Besides, the expression of chitinase gene was induced by in a RERJ1-dependent manner in the transgenic rice in which RERJ1 expression could be controlled by an estrogen treatment. Here, we identified JA-responsive cis element in the promoter region of the chitinase by reporter-gene assay. We conducted the deletion analysis of the chitinase promoter regions located from -2415 to -265 bp upstream of the translation start site. Deletions from -2415 to -515 bp did not have a significant effect on responsiveness to JA, but a further deletion to -265 bp rendered the promoter incapable of responding to JA. This result suggests that the region from -515 to -265 bp contains JA-responsive cis element. Indeed, the region contains three E-box elements, which are putative binding sites for bHLH transcription factors. The mutation analysis showed that an E-box at -448 to -443 bp is essential for responding to JA. Direct binding of RERJ1 to the E-box is now being examined.
収録刊行物
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- 植物化学調節学会 研究発表記録集
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植物化学調節学会 研究発表記録集 42 (0), 43-, 2007
一般社団法人 植物化学調節学会
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詳細情報 詳細情報について
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- CRID
- 1390001204423818624
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- NII論文ID
- 110006474723
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- NII書誌ID
- AA11550064
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- ISSN
- 21896313
- 09191887
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可