34. Regulation of photoperiodic flowering related gene expression by DNA methylation
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- Kondo Hiroshi
- Grad. Sch. Sci. Tech., Niigata Univ.:JSPS Research Fellow
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- Kato Akira
- Grad. Sch. Sci. Tech., Niigata Univ.:Dept. Biol., Fac. Sci., Niigata Univ.
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- Itoh Kimiko
- Grad. Sch. Sci. Tech., Niigata Univ.
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- Okazaki Keiichi
- Dept. Agrobiology, Fac. Agr., Niigata Univ.
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- Takeno Kiyotoshi
- Grad. Sch. Sci. Tech., Niigata Univ.:Dept. Biol., Fac. Sci., Niigata Univ.
Bibliographic Information
- Other Title
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- 34. DNAメチル化による光周的花成関連遺伝子の発現制御(口頭発表)
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Abstract
We found that the treatment with a DNA demethylating reagent, 5-azacytidine (azaC) induced flowering in Perillafrutescens, suggesting that the flowering of P. frutescens was epigenetically regulated. The progeny of the plants flowered by azaC treatment did not flower under non-inductive photoperiod. The flowering-related genes activated by azaC treatment may be deactivated by remethylation during the generation change. Thus, we hypothesized that the expression of flowering-related genes is regulated by DNA methylation. Accordingly, we compared the genomic DNA extracted from photoperiodically induced P. frutescens and that from non-induced control plants by MS-AFLP technique, and detected some fragments specific to the DNA sample of photoinduced plants. This suggests that the inductive photoperiod changed DNA methylation status. Some flowering-specific fragments were cloned, and some of them contained CpG island-like regions which are known to play an important role in epigenetical regulation in mammals. We found a fragment homologous to the gene encoding Zn-finger domain-containing protein of rice. Zn-finger domain is contained in the protein important to vernalization, suggesting a relationship to epigenetics. Interestingly, this fragment had a CpG island-like region. These results suggest that the photoperiodic flowering of P.frutescens may be regulated by DNA methylation in CpG islands and the genes for Zn-finger containing proteins.
Journal
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- The Janapese Society for Chemical Regulation of Plants, Abstract
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The Janapese Society for Chemical Regulation of Plants, Abstract 42 (0), 47-, 2007
The Janapese Society for Chemical Regulation of Plants
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Details 詳細情報について
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- CRID
- 1390282679400524032
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- NII Article ID
- 110006474727
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- NII Book ID
- AA11550064
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- ISSN
- 21896313
- 09191887
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed