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Abstract
レタスの系統維持のため花茎の節培養を行った.レタスとL. serriolaの雑種およびレタスの四倍体は34から40節で花芽を形成し,播種後110日あるいは140日にはそれぞれの株の頂部から順に開花した.2次花茎の節の外植体からの葉芽形成率は四倍体レタスでは44.8%,L. serriola×レタスでは19.9%であったが,これは四倍体レタスの方が発育は遅く,外植体における花芽の形成が進んでいなかったためと考えられた.花芽発育ステージの遅い主茎の低い節位から発生した2次花茎の外植体も上位の節位の外植体より葉芽形成率が高かった.また,主茎の頂部の頭花が開花した株の2次花茎の節の外植体の葉芽形成率は5.6%と低く,発育ステージが遅く頭花の未開花の外植体のそれは55.1%と高かった.さらに,2次花茎の節位が主茎側に近いものほど葉芽形成率が高かった.得られた葉芽は移植後,発根し,馴化した後に,開花結実した.本研究で明らかになった抽だい開花しているレタスの花茎の節を培養して,葉芽形成を誘導する方法は,種子を生産した後においても,その個体を維持することに役立つものと思われた.
Nodal culture of flower stalk was carried out to maintain the breeding line of lettuce. The flower buds of lettuce and its interspecific hybrids with Lactuca serriola and tetraploid lettuce were initiated on 40th to 34th node of main stem and bloomed at 140 to 110 days after sowing. The explants from node of secondary stem of tetraploid lettuce and L. serriola×lettuce proliferated leaf buds in a rate of 44.8 and 19.9%, respectively, indicating that the explants from the developed stage of flower seemed to form flower bud rather than leaf bud or shoot. Then, the explants of secondary stem from the lower node of the main stem showed higher shoot formation than those from the upper node. When the terminal flower of the main stem bloomed, the percentage of explants which formed leaf buds (5.6%) was less than that of none-bloomed explants (55.1%). Explants from lower node of secondary stalk formed leaf bud more frequently than those of upper node. This study enables the maintenance of the plants on breeding lines of lettuce even after the seed production by node culture of flower stalk.
Journal
- Horticultural research (Japan) [List of Volumes]
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Horticultural research (Japan) 7(1), 17-21, 2008-01-15 [Table of Contents]
The Japanese Society for Horticultural Science