口腔白板症上皮細胞へのマイコプラズマの侵入

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  • Invasion of mycoplasma in epithelial cells of oral leukoplakia

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Oral leukoplakia is the most common premalignant lesion of the oral mucosa, although its etiology has not yet been elucidated. Hoechst 33258, DNA binding fluorescent dye, staining of epithelial cells from oral leukoplakia tissues has revealed small, granular, and fluorescent structures in the cytoplasm, which are suggestive of microorganism infection. The Hoechst 33258-stained particles have been speculated to be mycoplasmas. Some mycoplasmas, such as Mycoplasma salivarium and Mycoplasma orale, form part of normal flora of the oral cavity, and can be detected in dental plaque and saliva. So, this study was performed to examine the location of mycoplasma in the epithelial cells of oral leukoplakia tissue by immunohistochemistry, immunoelectron microscopy, nested polymerase chain reaction (PCR) and sequencing of nested PCR products. By immunohistochemistry, small, granular fluorescent structures in the cytoplasm of epithelial cells were observed. The results of immunoelectron microscopy demonstrated the presence of small (0.5-0.8μm in size) polymorphous, electron dense structures with definite borders. Their inner structures were relatively homogeneous and their borders were definite. And binding of gold particles was observed limitedly on the structures. The results of nested PCR and sequencing of PCR products showed the presence of M. salivarium in the epithelial cells of three leukoplakia tissues. From these findings, invasion of M. salivarium in the oral mucosal epithelial cells of leukoplakia is shown.

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