Effects of perfluorooctane sulfonate (PFOS) on swimming behavior and membrane potential of paramecium caudatum
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- Kawamoto Kosuke
- Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University Department of Applied Veterinary Science, The Doctoral Course of the United Graduate School of Veterinary Science, Gifu University
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- Nishikawa Yasuo
- Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
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- Oami Kazunori
- Graduate School of Life and Environmental Sciences, University of Tsukuba
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- Jin Yihe
- Department of Environmental Science and Technology, Dalian University of Technology
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- Sato Itaru
- Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
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- Saito Norimitsu
- Research Institute for Environmental Sciences and Public Health of Iwate Prefecture
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- Tsuda Shuji
- Laboratory of Veterinary Public Health, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
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Persistent perfluorinated organic compounds such as perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) were distributed widely in the global. PFOS (15 µM or higher) caused backward swimming of paramecia. The Triton-extracted paramecia, where the membrane was disrupted and the externally applied chemicals are freely accessible to the ciliary apparatus, showed forward swimming up to 0.1 µM Ca2+ in the medium and backward swimming at about 0.2 µM and higher. PFOS (0.1 mM) did not change the relationship between the swimming directions and free Ca2+ concentrations. Effects of various surfactants including PFOS and PFOA on the swimming direction of paramecia were compared with the hemolysis of mouse erythrocytes as an indicator of surfactant activities. The hemolysis did not correlate with their swimming behavior. PFOS caused triphasic membrane potential changes both in the wild-type paramecia and caudatum non-reversal (CNR) mutants, the latter is defective in voltage-gated Ca2+ channels. An action potential of the wild-type specimen was induced at lower current intensity when PFOS was present in the medium. Voltage-clamp study indicated that PFOS had no effect on the depolarization-induced Ca2+ influx responsible for the action potential. The membrane potential responses obtained were similar to those obtained by the application of some bitter substances such as quinine that activate chemoreceptors of paramecia. Since the CNR specimens did not exhibit PFOS-induced backward swimming at concentrations examined, the backward swimming is attributable to the influx of Ca2+ into the cilia through voltage-gated Ca2+ channels. The Ca2+ channels are most probably activated by the depolarizing receptor potentials resulted from the PFOS-induced activation of chemoreceptors.
収録刊行物
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 33 (2), 155-161, 2008
一般社団法人 日本毒性学会
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詳細情報 詳細情報について
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- CRID
- 1390282679877337344
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- NII論文ID
- 110006665965
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- NII書誌ID
- AN00002808
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- ISSN
- 18803989
- 03881350
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- NDL書誌ID
- 9513814
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可