ダイエットを目的とした健康食品中に含まれる医薬品成分の液体クロマトグラフィー/タンデム質量分析計(LC/MS/MS)による迅速分析法  [in Japanese] Rapid Determination of Medical Components Found in the Health Food for Weight Loss by Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)  [in Japanese]

A method for the rapid determination of 11 medical components found in health foods for weight loss using liquid chromatography/tandem mass spectrometry (LC/MS/MS) has been developed. HPLC separation is performed on an ODS column with the gradient elution method. The mobile phase consists of two solvents. Solvent A is water and solvent B is methanol/acetonitrile (1:1), and both contain 0.1% formic acid and 5mmol/l of ammonium acetate. Each medical component is analyzed with multiple-reaction monitoring (MRM) in both negative and positive modes through electrospray ionization (ESI). The recovery rates of the 11 medical components added to commercially available health foods were 46.3-114% and each coefficient of variation was 13.7% or less. It was confirmed that this method is applicable to the urgent analysis of health foods that have caused damage to health.