70(P1-18) 固定化植物培養細胞による外来有機物質の生物変換(ポスター発表の部)  [in Japanese] 70(P1-18) Biotransformation of Organic Foreign Substrates with Immobilized Plant Cell Cultures  [in Japanese]

Immobilized plant cell cultures such as Nicotiana tabacum and Gardenia jasminoides Ellis entrapped with calcium alginate beads have been prepared and the biotransformation of synthetically important foreign substrates was examined by consecutively reusing these two immobilized cells. Immobilized cells of N. tabacum were reusable several times and enantioselectively reduced the five keto esters 1-5 to the corresponding hydroxy esters 1a-5a in reasonable chemical yields and relatively high optical yields. The consecutive reuse of the immobilized cells enhanced the rates of each biotransformation of 1-5. The time-course experiments of the present biotransformations clearly revealed that the transformation rates of 3-oxobutanoates 3 and 4, both of which bear longer n-alkyl chains in their alkoxycarbonyl groups, were faster than those of homologs 1 and 2 possessing shorter alkyl chains. The biotransformation of an aromatic ketone, acetophenone (6), with immobilized N. tabacum cells gave a chiral alcohol (S)-(-)-6a, whereas a similar one with immobilized G. jasminoides cells yielded (R)-(+)-6a.