Atorvastatin Downregulates BMP-2 Expression Induced by Oxidized Low-Density Lipoprotein in Human Umbilical Vein Endothelial Cells
-
- Zhang Ming
- Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha
-
- Zhou Sheng-Hua
- Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha
-
- Li Xu-Ping
- Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha
-
- Shen Xiang-Qian
- Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha
-
- Fang Zhen-Fei
- Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha
-
- Liu Qi-Ming
- Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha
-
- Qiu Shuang-Fa
- Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha
-
- Zhao Shui-Ping
- Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha
Search this article
Abstract
Background Bone morphogenetic protein-2 (BMP-2) plays a key role both in vascular development and pathophysiological processes. However, the effects of oxidized low-density lipoprotein (ox-LDL) combined with atorvastatin on BMP-2 expression are entirely unknown in human umbilical vein endothelial cells (HUVECs). The present study investigates the effects of ox-LDL on BMP-2 expression. Furthermore, the influence of atorvastatin on ox-LDL-induced BMP-2 expression is also examined. Methods and Results The HUVECs were treated by ox-LDL or combined with pyrrolidine dithiocarbamate (PDTC) or atorvastatin. The expression level of BMP-2 mRNA was examined by real-time PCR and RT-PCR analysis. The expression of BMP-2 protein was assayed by enzyme-linked immunosorbent assay. The malondialdehyde (MDA) and activities of total superoxide dismutase (SOD) were detected by routine methods. The activation of nuclear factor κB (NF-κB) in HUVECs was determined using an assay kit from active motif and western blot analysis. Ox-LDL treatment significantly increased BMP-2 expression, which is associated with NF-κB activation, but BMP-2 expression was suppressed by treatment with PDTC or atorvastatin. Furthermore, the increase in MDA levels and decrease in activities of total SOD caused by ox-LDL treatment were reversed by the treatment of PDTC or atorvastatin. Conclusions Ox-LDL-induced BMP-2 expression was suppressed by PDTC or atorvastatin treatment. The effects of atorvastatin might contribute to the mechanisms by inhibiting NF-κB activation. (Circ J 2008; 72: 807 - 812)<br>
Journal
-
- Circulation Journal
-
Circulation Journal 72 (5), 807-812, 2008
The Japanese Circulation Society
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282680079177088
-
- NII Article ID
- 110006680562
-
- NII Book ID
- AA11591968
-
- COI
- 1:STN:280:DC%2BD1c3nvVOmtA%3D%3D
-
- ISSN
- 13474820
- 13469843
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed