30 ドキソルビシン生合成中の環化酵素DnrD : 大腸菌での大量発現、精製およびその性質(口頭発表の部)
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- 片山 欣哉
- ウィスコンシン大・薬
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- Kendrew Steven G.
- ウィスコンシン大・薬
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- Deutsch Eric
- ウィスコンシン大・薬
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- Madduri Krishna
- ウィスコンシン大・薬
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- Thompson Thomas B.
- ウィスコンシン大・酵素研、生化
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- Rayment Ivan
- ウィスコンシン大・酵素研、生化
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- Hutchinson C. Richard
- ウィスコンシン大・薬、細菌
書誌事項
- タイトル別名
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- 30 The DnrD Cyclase Involved in the Biosynthesis of Doxorubicin : Purification and Characterization of the Recombinant Enzyme
抄録
Mutations in the Streptomyces peucetius dnrD gene block the ring cyclization leading from aklanonic acid methyl ester to aklaviketone, an intermediate in the biosynthetic pathway to daunorubicin and doxorubicin. To investigate the role of DnrD in this transformation its gene was overexpressed in Escherichia coli and the DnrD protein was purified to homogeneity and characterized. The enzyme was shown to catalyse the conversion of aklanonic acid methyl ester to aklaviketone presumably via an intramolecular aldol condensation mechanism. In contrast to the analogous intramolecular aldol cyclization catalyzed by the TcmI protein from the tetracenomycin C pathway in Streptomyces glaucescens, where a tricyclic anthraquinol carboxylic acid is converted to its fully aromatic tetracyclic form, the conversion catalyzed by DnrD occurs after anthraquinone formation and requires activation of a carboxylic acid group by esterification of aklanonic acid, the aklanonic acid methyl ester precursor. Also, the cyclization is not coupled with a subsequent dehydration step that would result in an aromatic ring. As the substrates for the DnrD and TcmI enzymes are among the earliest isolable intermediates of aromatic polyketide biosynthesis, an understanding of the mechanism and active site topology of these proteins will allow one to determine the substrate and mechanistic parameters important for aromatic ring formation. In the future, these parameters may be able to be applied to some of the earlier polyketide cyclization processes that currently are difficult to study in vitro.
収録刊行物
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- 天然有機化合物討論会講演要旨集
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天然有機化合物討論会講演要旨集 41 (0), 175-180, 1999
天然有機化合物討論会実行委員会
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詳細情報
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- CRID
- 1390282681056532736
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- NII論文ID
- 110006681825
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- ISSN
- 24331856
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可