75(P-67) Studies on the Constituents from the Fruits of Vitex rotundifolia
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- Ono Masateru
- School of Agriculture, Kyushu Tokai University
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- Masuoka Chikako
- School of Agriculture, Kyushu Tokai University
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- Ito Yasuyuki
- School of Agriculture, Kyushu Tokai University
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- Yoshimitsu Hitoshi
- Faculty of Engineering, Kyushu Kyoritsu University
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- Nohara Toshihiro
- Faculty of Pharmaceutical Sciences, Kumamoto University
Bibliographic Information
- Other Title
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- 75(P-67) 蔓荊子の成分に関する研究(ポスター発表の部)
Abstract
Vitex rotundifolia L. (Verbenaceae) is widely distributed in Asia, and its fruit, Viticis Fructus is used as folk medicine for headaches, colds, eye-pain etc. During the course of our studies on natural antioxidants from crude drugs, the methanol extract of this fruit showed a stronger antioxidative activity than 3-tert-butyl-4-hydroxyanisole (BHA), which is the standard synthetic antioxidant, using the ferric thiocyanate method. Therefore, we have examined the constituents of the methanol extract, and isolated twenty-three labdane-type (1-23), a rearranged labdane-type (24), four norlabdane-type (25-28), two abietane-type (29, 30) and a rearranged abietane-type (31) diterpenes, eight iridoids (32-39) and eleven aromatic compounds (40-50). Among them, thirty compounds (1-21, 23, 24, 26-28, 31, 38, 39, 48) are regarded as novel ones. The structures were characterized on the basis of spectroscopic data and X-ray crystallographic analysis. Of the isolated compounds, fifteen compounds (6, 29, 30, 36, 37, 40-43, 45-50) were examined for their antioxidative activity using the ferric thiocyanate method, and eleven compounds (30, 37, 40-43, 45-49) indicated a stronger antioxidative activity than the standard natural antioxidant, α-tocopherol. Especially, nine compounds (30, 37, 40-43, 45-47) were identified as stronger antioxidants than BHA. Moreover, the scavenging effect of thirteen compounds (30, 36, 37, 40-43, 45-50) on the stable radical 1,1-dipheny1-2-picrylhydrazyl (DPPH) was examined. Among them, six compounds (40-43, 45, 46) exhibited potent scavenging effect. In particular, the effect of one dihydroflavonol (46) was almost twice that of α-tocopherol at a concentration of 0.02mM. In addition, eight compounds (1-6, 8, 11) were investigated for their immunosuppressive activity on mouse allogenic mixed lymphocyte reaction (MRL) in vitro, and all tested compounds showed the activity (IC_<50>: 1, 21.07μM; 2, 13.85μM; 3, 126.37μM; 4, 58.71μM; 5, 98.09μM; 6, 11.43μM; 8, 22.70μM; 11, 17.72μM).
Journal
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- Symposium on the Chemistry of Natural Products, symposium papers
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Symposium on the Chemistry of Natural Products, symposium papers 42 (0), 445-450, 2000
Symposium on the Chemistry of Natural Products Steering Committee
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Details 詳細情報について
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- CRID
- 1390282681054975616
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- NII Article ID
- 110006681977
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- ISSN
- 24331856
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed