107(P-50) Cellular localization of dysiherbaine in the producing organism
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- Sakai Ryuichi
- Kitasato University School of Fisheries Sciences
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- Yoshida Kazufumi
- Kitasato University School of Fisheries Sciences
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- Koike Kanae
- Kitasato University School of Fisheries Sciences
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- Jimbo Mitsuru
- Kitasato University School of Fisheries Sciences
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- Koike K.
- Kitasato University School of Fisheries Sciences
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- Minato Sakiko
- Kitasato University School of Fisheries Sciences
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- Kamiya Hisao
- Kitasato University School of Fisheries Sciences
Bibliographic Information
- Other Title
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- 107(P-50) 興奮性アミノ酸ダイシハーベインの組織内局在性 : 産生生物と存在意義の解明に向けて(ポスター発表の部)
Abstract
Cellular localization of the marine sponge-derived excitatory amino acid dysiherbaine (1) was investigated using immunohistochemical methods. Dysiherbaine (1), isolated from a sponge Dysidea herbacea, selectively activates mammalian non-NMDA type glutamate receptors (GluRs) and induces seizure in mice. Recently 1 was found to have unusually high affinity towards GluR5 and 6, subtypes of kainite-type GluRs, and can activate these subtype receptors selectively in the heteromerically expressed receptor complex. These unusual actions of 1 to the neuronal receptors warrants its usefulness as a research tool, however, its production, biosynthesis, and roles in the natural environment are left to be investigated. Since microorganisms including a cyanobacterial, Oscillatoria spongeliae are known to be heavily associated with D. herbacea, actual producers of the secondary metabolites and their roles in the sponge and associated microorganisms are difficult problem to solve. In this study, we paved a way to approach this elusive problems by showing cellular localization of 1 using immnumohistochemical methods. We also show a presence of putative DH-binding protein of 111kD using DH-conjugated affinity chromatography and SDS-PAGE.
Journal
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- Symposium on the Chemistry of Natural Products, symposium papers
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Symposium on the Chemistry of Natural Products, symposium papers 45 (0), 629-634, 2003
Symposium on the Chemistry of Natural Products Steering Committee
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Details 詳細情報について
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- CRID
- 1390001206079932288
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- NII Article ID
- 110006682366
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- ISSN
- 24331856
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed