P-37 海洋糸状菌代謝物の絶対配置に関する研究(ポスター発表の部) [in Japanese] P-37 Study on Absolute Stereostructures of Metabolites from Marine Fungus [in Japanese]
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A growing number of marine fungi have been reported to produce novel and potentially life-saving bioactive secondary metabolites. Herein, we report chemical investigation of the metabolites obtained from the extracts of some sorts of marine fungi culture filtrate. Mycosphaerella molleriana was cultured in 1/2 PD 50% sea water for 19 days under 20℃. We succeeded in isolation of a new compound (1) from the EtOAc extract. The absolute configuration of 1 was elucidated from the measurement of CD spectrum of its benzoated. Criptosphaeria eunomia var. eunomia was cultured in 1/2 PD 50% sea water for 4 weeks under 20℃. A new compound (2) from the EtOAc extract as well as two known compounds (3,4) were obtained. These compounds were recrystallized from CHCl_3 to give solvated crystals and their absolute configurations were finely determined by X-ray. We previously reported three antibacterial chlorine-containing components of A. ostianus strain 01F313 that had been isolated from an unidentified marine sponge collected at Pohnpei. Expecting that bromine-containing compounds might be obtained when a medium composed of a bromide solution in place of seawater was used, we cultivated the same strain in a bromine-modified 1/2 PD medium. Although we were unable to isolate the brominated compounds, we found that the metabolites were considerably different from those obtained from the strain cultured in the seawater medium, and succeeded in isolating six new compounds (5,6,7,10,11 and 12) as well as two known compounds 8 and 9. We elucidated the structures of the new compounds and the absolute configurations of the obtained compounds by the application of the modified Mosher's method.