2,4-Dichlorophenoxyacetic Acid (2,4-D)- and 2,4,5-Trichlorophenoxyacetic Acid (2,4,5-T)-Degrading Bacterial Community in Soil-Water Suspension during the Enrichment Process

• Huong Nguyen L.

  Faculty of Life and Environmental Science, Shimane University

• Itoh Kazuhito

  Faculty of Life and Environmental Science, Shimane University

• Suyama Kousuke

  Faculty of Life and Environmental Science, Shimane University

抄録

Changes in the bacterial community in soil-water suspensions during the enrichment period of 2,4-D and 2,4,5-T degraders were examined using denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA gene. The nucleotide sequences of almost all major bands at the degradation of 2,4-D and 2,4,5-T corresponded to those of 2,4-D- and 2,4,5-T-degrading isolates, and successions of diverse 2,4-D- and 2,4,5-T-degrading bacterial communities were demonstrated in the DGGE profile. These results suggested that 2,4-D- and 2,4,5-T-degrading isolates were responsible for the degradation of 2,4-D and 2,4,5-T in soil-water suspensions; however, one major band of a non-degrader was found in some cases. Therefore, to elucidate the relationship between the structures and functions of bacterial communities, the importance of a combined approach using both culture-dependent and -independent methods was demonstrated.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 23 (2), 142-148, 2008

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• 2,4-D
• 2,4,5-T
• degrading bacterial community
• PCR-DGGE

詳細情報

• CRID: 1390001204345867136
• NII論文ID: 110006684365
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.23.142
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 9510837
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9510837
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/23/2/23_2_142/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可