セレノシステイン生合成系とその種多様性

DOI Web Site Web Site 参考文献46件
  • 水谷 隆治
    名古屋市立大学大学院薬学研究科医薬品代謝解析学分野
  • 大坂 享史
    名古屋市立大学大学院薬学研究科医薬品代謝解析学分野
  • 藤原 俊伸
    神戸大学大学院工学研究科
  • Shahidzzman M.
    名古屋市立大学大学院薬学研究科医薬品代謝解析学分野

書誌事項

タイトル別名
  • Biochemical Selenosysteine Synthesis and the Phylogenic Study
  • セレノシステイン セイゴウセイケイ ト ソノ シュ タヨウセイ

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  Selenium (Se) is an essential trace element. Se is found as selenocysteine (Sec) in Se-proteins. Sec is the 21st amino acid, because Sec has its tRNA, the codon UGA and those components in its translational machinery. Sec UGA codon shares with major stop codon UGA. We purified Sec synthesizing enzymes, such as seryl-tRNA synthetase (SerRS), Sec synthetase (SecS) and selenophosphate synthetase (SePS). I described the procedures to prepare Sec tRNA, SerRS, SecS, SePS and [75Se]H2Se in detail. We clarified that SecS composed of two proteins, SecSα and SecSβ. Sec synthesizing and incorporating systems present in Monela, Animalia and Protoctista but not in Plantae and Fungi. We showed that protozoa had Sec tRNA on which Sec was synthesized from Ser-tRNA by bovine and protozoa SecS. Some worms, such as Caenorhabditis elegans and Fasiola gigantica, also had Sec tRNA on which Sec was synthesized by bovine liver SecS or C. elegans enzymes. We showed recognition sites of mammalian Sec tRNA by SecS. The identitiy units of Sec tRNA are 9 bp aminoacyl- and 6 bp D-stems. This recognition is not the base-specific manner but the length-specific manner. From comparison of the phylogeny trees of Sec synthesizing system and translation system, we concluded that the evolution of Sec synthesizing system is older than that of the translation system.<br>

収録刊行物

  • 薬学雑誌

    薬学雑誌 128 (7), 989-996, 2008-07-01

    公益社団法人 日本薬学会

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