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This research involves the development of a new approach to elucidate the health influence on humans by exposure to nanoparticles. Detailed information was obtained on the shape, particle diameter, composition of particulates in a single cell and a method was found to correlate that information to the biological response of the cell. This is a key technique to clarify the physical and chemical properties of nanoparticles, which will have a fatal influence on the human body. First, cellular oxidative stress by exposure to diesel exhaust particles was investigated by using a time-of-flight secondary ion mass spectrometry(TOF-SIMS) and a real time reverse transcription polymerase chain reaction(RT-PCR) and a scanning transmission electron microscope(STEM). From these results, it was clear that there was interaction between the cellular functions and the particle properties. Next, nanoparticles in the atmospheric environment neighboring a traffic route were investigated by using a differential mobility analyzer(DMA) and TOF-SIMS. From these results, it was revealed that there were different types (particle diameter ; 20 nm or 100 nm) of environmental nanoparticles. Thus, it is very important for this research to improve both analytical and biological techniques.
