スクアレン環化酵素の生合成工学

• 阿部 郁朗

  静岡県立大学薬学部 科学技術振興機構PRESTO

書誌事項

タイトル別名

• Engineering of Squalene Cyclizing Enzymes
• スクアレン カンカ コウソ ノ セイゴウセイ コウガク

抄録

  The broad substrate tolerance and catalytic potential of squalene cyclizing enzymes of bacterial and plant origin are remarkable; the enzymes readily accept variety of non-physiological substrate analogues and efficiently perform sequential ring-forming reactions to produce a series of unnatural cyclic triterpenes. By utilizing the catalytic plasticity of the enzymes, it is possible to generate unnatural novel cyclic polyprenoids by enzymatic conversion of chemically synthesized substrate analogues. Here we present recent examples including (a) enzymatic formation of a “supra-natural” hexacyclic polyprenoid as well as heteroaromatic ring containing cyclic polyprenoids by bacterial squalene: hopene cyclase from Alicyclobacillus acidocaldarius, and (b) enzymatic cyclization of 22,23-dihydro-2,3-oxidosqualene and 24,30-bisnor-2,3-oxidosqualene by plant oxidosqualene: β-amyrin cyclase from Pisum sativum.<br>

収録刊行物

• 薬学雑誌

  薬学雑誌 128 (8), 1109-1118, 2008-08-01

  公益社団法人 日本薬学会

キーワード

• squalene cyclase
• oxidosqualene cyclase
• triterpenes
• enzyme
• substrate specificity
• engineered biosynthesis

詳細情報

• CRID: 1390282681103585920
• NII論文ID: 110006834237
• NII書誌ID: AN00284903
• DOI: 10.1248/yakushi.128.1109
• COI: 1:STN:280:DC%2BD1cvptVSjsw%3D%3D
• ISSN: 13475231
• ISSN: 00316903
• NDL書誌ID: 9603286
• Web Site: http://id.ndl.go.jp/bib/9603286
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9603286
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/128/8/128_8_1109/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可