1α,25-dihydroxyvitamin D3-26,23-lactam, a novel vitamin D3 analog, acts as a vitamin D3 antagonist in human prostate cancer cells

DOI Web Site Web Site 参考文献36件
  • Takita Morichika
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • Hirata Michiko
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • Tsukamoto Kazuki
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • Nagasawa Kazuo
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • Miyaura Chisato
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • Inada Masaki
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

書誌事項

タイトル別名
  • 1.ALPHA.,25-Dihydroxyvitamin D3-26,23-lactam, a Novel Vitamin D3 Analog, Acts as a Vitamin D3 Antagonist in Human Prostate Cancer Cells
  • 1 a 25 dihydroxyvitamin D3 26 23 lactam a novel vitamin D3 analog acts as a vitamin D3 antagonist in human prostate cancer cells

この論文をさがす

抄録

1α,25-Dihydroxyvitamin D3 [1α,25(OH)2D3], the active form of vitamin D3, is known to exhibit an anti-tumor properties and markedly suppresses the growth of various human cancer cells. We synthesized novel vitamin D analogs, 1α,25-dihydroxyvitamin D3-26,23-lactam (DLAMs), having a lactam moiety in the side chain, and examined the effects on cell growth of human prostate cancer cells LNCaP. 1α,25(OH)2D3 significantly suppressed both the number of cells and cell viability. The mRNA expression of p21, well-known as a tumor suppressive gene, was clearly induced by treatment with 1α,25(OH)2D3 in LNCaP cells. The effects of 1α,25(OH)2D3 on the growth suppression of LNCaP cells was attenuated by the simultaneous addition of (23S,25S)-DLAM-1P. In a computer docking simulation, (23S,25S)-DLAM-1P bound to vitamin D receptor (VDR), and its lactam moiety may interfere VDR helix-12 folding. Its stereoisomer (23R,25R)-DLAM-1P did not influence cell growth regulated by 1α,25(OH)2D3. The expression of p21 mRNA induced by 1α,25(OH)2D3 was suppressed by (23S,25S)-DLAM-1P but not by (23R,25R)-DLAM-1P in LNCaP cells. In the absence of 1α,25(OH)2D3, neither (23S,25S)-DLAM-1P nor (23R,25R)-DLAM-1P regulated the cell growth of LNCaP cells. Thus, (23S,25S)-DLAM-1P interferes with the VDR signal and acts as a vitamin D3 antagonist in cancer cells.

収録刊行物

参考文献 (36)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ