Bacteriology: Cultivation and characterization of Lawsonia intracellularis isolated from rabbit and pig
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- WATARAI Masahisa
- Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
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- YOSHIYA Masahiro
- Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine
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- SATO Akane
- Department of Pathological Science, Obihiro University of Agriculture and Veterinary Medicine
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- FURUOKA Hidefumi
- Department of Pathological Science, Obihiro University of Agriculture and Veterinary Medicine
書誌事項
- タイトル別名
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- Cultivation and Characterization of Lawsonia intracellularis Isolated from Rabbit and Pig
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抄録
Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. In this study, we improved the in vitro cultivation method of L. intracellularis to increase the passage efficiency and showed that L. intracellularis isolated from a rabbit and a pig have different antigenic properties. Bacteria should be recovered from infected cells before cell death due to infection to obtain higher bacterial passage efficiency, and measurement of LDH activity in the cell culture medium was useful for determining the timing of bacterial passage. L. intracellularis isolated from the rabbit and pig showed different band patterns in immunoblotting. Our results should be helpful in the development of serological diagnosis and epidemiological investigation methods.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 70 (7), 731-733, 2008
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681406509568
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- NII論文ID
- 110006839598
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BD1crgtlSktw%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 9591649
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可