Peroxidase-Like Catalytic Activity of Aqueous- and Immobilized-Mn3+-octabromo-porphyrins on Ion-Exchange Resin Supplied as Mimetic of Horseradish Peroxidase

• Kitamura Youji

  Department of Pharmaceutical Sciences, Graduate School of Medicine and Dentistry and Pharmaceutical Sciences, Okayama University

• Mori Katsuya

  Department of Pharmaceutical Sciences, Graduate School of Medicine and Dentistry and Pharmaceutical Sciences, Okayama University

• Yamamoto Makiko

  Department of Pharmaceutical Chemistry, Graduate School of Natural Science and Technology, Okayama Universi-ty

• Nozaki Akira

  Department of Pharmaceutical Chemistry, Graduate School of Natural Science and Technology, Okayama Universi-ty

• Saito Madoka

  Faculty of Pharmaceutical Sciences, Mukogawa Women's University

• Tsukamoto Ikuko

  Faculty of Medicine, Kagawa University

• Mifune Masaki

  Department of Pharmaceutical Sciences, Graduate School of Medicine and Dentistry and Pharmaceutical Sciences, Okayama University

• Saito Yutaka

  Department of Pharmaceutical Sciences, Graduate School of Medicine and Dentistry and Pharmaceutical Sciences, Okayama University

抄録

In order to explore the capability of metal porphyrins as an alternative of horseradish peroxidase (HRP), HRP-like activi-ty of three manganese-porphyrins (Mn-Ps) and three Mn-octabromo-porphyrins (Mn-OBPs) was examined in both aqueous and immobilized states. It was found that Mn³⁺-octabromotetrakis(1-methyl-pyridinium-4yl)porphine (Mn-OBTMPyP) has an activity of at least 90% of HRP in an aqueous solution. Mn-OBTMPyP exhibited a catalytic activity even in the presence of hydrogen peroxide without suicide reaction. In addition, Mn-OBTMPyP was revealed to function as an alternative to HRP in the quantitative determination of serum uric acid. These results are of great interest because they indicate that metal-octabromo-porphyrins possibly include promising candidates of artificial enzyme capable of substituting for HRP.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 56 (9), 1364-1366, 2008

  公益社団法人 日本薬学会

キーワード

• horseradish-peroxidase
• mimetic
• manganese-porphyrin
• bromination

詳細情報

• CRID: 1390001204169454080
• NII論文ID: 110006878920
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.56.1364
• ISSN: 13475223
• ISSN: 00092363
• NDL書誌ID: 9622439
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9622439
• Web Site: http://www.jstage.jst.go.jp/article/cpb/56/9/56_9_1364/_pdf
• 本文言語コード: en
• データソース種別:
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