LacZマーカーレスキューアッセイ法によるRD114ウイルス検出法の確立

• SAKAGUCHI Shoichi

  Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University School of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine

• OKADA Masaya

  Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University

• SHOJIMA Takayuki

  Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University

• BABA Kenji

  Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University

• MIYAZAWA Takayuki

  Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University

書誌事項

タイトル別名

• Establishment of a LacZ Marker Rescue Assay to Detect Infectious RD114 Virus
• Virology: Establishment of a LacZ marker rescue assay to detect infectious RD114 virus

抄録

Cats have an infectious endogenous retrovirus, named RD114 virus, and there is a possibility that RD114 virus has contaminated live attenuated vaccines, for which feline cells are used as a substrate. To monitor infectious RD114 virus in vaccines for cats, we developed a LacZ marker rescue assay to detect infectious RD114 virus. Among four human cell lines examined, TE671 cells (human rhabdomyosarcoma) were most susceptible to RD114 virus and supported RD114 replication efficiently. Infection was enhanced approximately 5 times by the addition of polybrene at concentrations of 2 to 8 μg/ml in the medium during viral adsorption. A 4-hr viral adsorption period was sufficient to obtain the maximum titer. By inoculating samples into TE671 cells transduced with the lacZ marker gene, the limiting diluted sample (i.e., less than 10 infectious units) was detected at 12 days post-inoculation by the LacZ marker rescue assay. Based on the results obtained in this study, we propose a standard protocol of the LacZ marker rescue assay to detect infectious RD114 virus.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 70 (8), 785-790, 2008

  公益社団法人 日本獣医学会

キーワード

• detection
• endogenous retrovirus
• RD114 virus

詳細情報

• CRID: 1390001206429271680
• NII論文ID: 110006878925
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.70.785
• ISSN: 13477439
• ISSN: 09167250
• ISSN: http://id.crossref.org/issn/09167250
• NDL書誌ID: 9625262
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030762090
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9625262
• Web Site: http://www.jstage.jst.go.jp/article/jvms/70/8/70_8_785/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009002596
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可