ヘパリン不純物のNMR分析法  [in Japanese] NMR Analysis of a Contaminant in Heparin  [in Japanese]

Recently, it has been reported that certain lots of heparin are associated with an acute, rapid onset of serious side effects indicative of allergic reaction, and ^1H NMR is one of the convenience but strong analytical methods to identify a contaminant in heparin. However, an NMR signal from the contaminant in some cases is overlapped with a satellite peak from heparin, leading a misunderstanding of the presence of the contaminant. Here, we show the satellite peak observed close to the NMR signal of the contaminant, and recommend the ^<13>C decoupling NMR to discriminate the satellite peak from the contaminant.