抄録
本実験では,トウガラシ'CH-19甘'および'京唐菜'茎葉中のNO産生阻害成分について調査した.'CH-19甘'の葉および果実を用いて,NO産生抑制活性の細胞検定を行った.葉のアセトン抽出物の複数の画分で強いNO産生抑制活性が認められた.カラムクロマトグラフィーにより葉のアセトン抽出物から,NO産生抑制成分の一つとしてアピゲニンを分離同定した.アピゲニンがポリフェノールの一種であることから,軟弱野菜用品種'京唐菜'の茎葉中のポリフェノール組成を調査した.'京唐菜'に含まれる主なポリフェノール類は,アピゲニン配糖体およびルテオリン配糖体であることが明らかになった.また,アピゲニン配糖体,ルテオリン配糖体の含量はそれぞれ平均227,0,29.3μmol・100g^<-1>FWであった.
The leaves of pepper plants contain abundant iron, calcium and vitamins. We have bred a pepper cultivar 'Kyotona'as a new leafy vegetable. In this report, nitric oxide (NO)-generation inhibitor was investigated in young pepper leaves and stems. Pepper leaves or fruits were tested for in vitro NO-generation inhibitory activities in a mouse macrophage RAW 264.7 cell. Acetone extracts from the leaves showed marked inhibitory activity on NO-generation. Then we tried to isolate NO-generation inhibitors from acetone extracts of leaves by column chromatographies. As a result, apigenin was isolated as one of the inhibitors of NO-generation. Major polyphenols in'Kyotona'were investigated by HPLC. It was demonstrated that major polyphenols in 'Kyotona' leaves and stems were apigenin glycosides and luteolin glycosides. The average contents of apigenin glycosides and luteolin glycosides were 29.3 and 227.0μmol・100g^<-1>FW, respectively.
