Edaravone (MCI-186) Scavenges Reactive Oxygen Species and Ameliorates Tissue Damage in the Murine Spinal Cord Injury Model

• AOYAMA Takeshi

  Department of Neurosurgery, Hokkaido University Graduate School of Medicine

• HIDA Kazutoshi

  Department of Neurosurgery, Hokkaido University Graduate School of Medicine

• KURODA Satoshi

  Department of Neurosurgery, Hokkaido University Graduate School of Medicine

• SEKI Toshitaka

  Department of Neurosurgery, Hokkaido University Graduate School of Medicine

• YANO Shunsuke

  Department of Neurosurgery, Hokkaido University Graduate School of Medicine

• SHICHINOHE Hideo

  Department of Neurosurgery, Hokkaido University Graduate School of Medicine

• IWASAKI Yoshinobu

  Department of Neurosurgery, Hokkaido University Graduate School of Medicine

抄録

The present study evaluated the effect of the free radical scavenger edaravone on lesion volume and neurological dysfunction after spinal cord injury (SCI) in mice, and investigated its protective effects on superoxide generation. Female C57BL/6 mice were subjected to SCI using a pneumatic impact device and were treated with 3 mg/kg of edaravone or vehicle 30 minutes before the insult. Motor functions were quantitatively evaluated. Lesion volume was assessed by Dohrmann’s two-cone method after one week. In situ detection of superoxide in the injured cord was carried out using the superoxide-sensitive dye dihydroethidium (DHE) staining technique. Pretreatment with edaravone significantly improved motor dysfunction and reduced the lesion volume to about 63% of the control (p < 0.05). Semi-quantitative measurements of red fluorescence emitted from DHE revealed that the superoxide concentration increased in the lesion periphery at 1 and 3 hours after the insult, and that pretreatment with edaravone significantly inhibited the increase of superoxide concentration in the lesion periphery at both time points (p < 0.0001). Double staining with DHE and monoclonal antibody against MAP2 showed that most cells positive for DHE were also positive for MAP2. These findings suggest that edaravone ameliorates tissue damage by scavenging reactive oxygen species, especially in the neurons, after SCI.<br>

収録刊行物

• Neurologia medico-chirurgica

  Neurologia medico-chirurgica 48 (12), 539-545, 2008

  一般社団法人 日本脳神経外科学会

キーワード

• dihydroethidium
• edaravone
• free radical
• secondary injury
• spinal cord injury
• superoxide

詳細情報

• CRID: 1390282680030590848
• NII論文ID: 110007008459
• NII書誌ID: AN00358613
• DOI: 10.2176/nmc.48.539
• ISSN: 13498029
• ISSN: 04708105
• Web Site: http://www.jstage.jst.go.jp/article/nmc/48/12/48_12_539/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009111408
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可