Virology: Recombination between vaccine and field strains of canine parvovirus is revealed by isolation of virus in canine and feline cell cultures

• MOCHIZUKI Masami

  Laboratory of Clinical Microbiology, Kyoritsu Seiyaku Corporation Advanced Technology Development Center, Kyoritsu Seiyaku Corporation

• OHSHIMA Takahisa

  Advanced Technology Development Center, Kyoritsu Seiyaku Corporation

• UNE Yumi

  Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University

• YACHI Akiko

  Laboratory of Clinical Microbiology, Kyoritsu Seiyaku Corporation

書誌事項

タイトル別名

• Recombination Between Vaccine and Field Strains of Canine Parvovirus is Revealed by Isolation of Virus in Canine and Feline Cell Cultures

抄録

Canine parvovirus type 2 (CPV) is a pathogen that causes severe hemorrhagic gastroenteritis with a high fatality rate in pups worldwide. Since CPV emerged in the late 1970s, its origin has been explored with the conclusion that CPV originated from feline panleukopenia virus or a closely related virus. Both high mutation rate and recombination are assumed to be key factors in the evolution of parvoviruses. Here we provide evidence for natural recombination in CPV isolated from dogs in cell culture. Antigenic and genetic properties of isolates from 10 diseased pups were elucidated. Six pups had been vaccinated beforehand with live combined vaccine containing original antigenic type CPV (CPV-2). Six isolates recovered from 4 vaccinated pups in cell cultures were found to contain either CPV-2 or CPV-2-like viruses. The other isolates, including all those from non-vaccinated pups, were CPV-2b viruses. Antigenic typing of two CPV-2-like isolates, 03-029/M and 1887/f, with a monoclonal antibody panel suggested they were a mixture of CPV-2 and CPV-2a (03-029/M) and a recombinant of CPV-2 and CPV-2b (1887/f). Genetic analysis of the VP1 gene indicated that isolate 03-029/M was a mixture of CPV-2, CPV-2a and a recombinant of CPV-2 and CPV-2a viruses, while isolate 1887/f was composed of a recombinant of CPV-2 and CPV-2b viruses. This is the first demonstration of natural CPV recombination in the field and suggests that recombination in the evolution of CPV is a more frequent and important process than previously believed.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 70 (12), 1305-1314, 2008

  公益社団法人 日本獣医学会

キーワード

• canine parvovirus
• cell culture
• dog
• parvovirus
• recombination

詳細情報

• CRID: 1390001206429162496
• NII論文ID: 110007021709
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.70.1305
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 9760147
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9760147
• Web Site: http://www.jstage.jst.go.jp/article/jvms/70/12/70_12_1305/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009123970
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可