Application of a Continuous Square-Wave Potential Program for Sub Nano Molar Determination of Ketotifen

• Daneshgar Parandis

  Center of Excellence in Electrochemistry, Department of Chemistry, University of Tehran

• Norouzi Parviz

  Center of Excellence in Electrochemistry, Department of Chemistry, University of Tehran Endocrinology & Metabolism Research Center, Tehran University of Medical Science

• Ganjali Mohammad Reza

  Center of Excellence in Electrochemistry, Department of Chemistry, University of Tehran Endocrinology & Metabolism Research Center, Tehran University of Medical Science

抄録

An electroanalytical method has been developed for the determination of the ketotifen by continuous square wave adsorptive stripping voltammetry on a ultra-gold microelectrode (Au UME) in aqueous solution with phosphate buffer as supporting electrolyte. The best adsorption conditions were found to be pH 2.3, an accumulation potential of 300 mV (Au vs. Ag: AgCl–KCl 3 M) and an accumulation time of 400 ms. Variation of admittance in the detection process is created by inhibition of oxidation reaction of the electrode surface, by adsorption of ketotifen. Furthermore, signal-to-noise ratio was significantly increased by application of discrete fast Fourier transform (FFT) method, background subtraction and two-dimensional integration of the electrode response over a selected potential range and time window. Also in this work some parameters such as square-wave frequency, eluent pH, and accumulation time were optimized. Effects of square-wave frequency, step potential and pulse amplitude were examined for the optimization of instrumental conditions. The calibration curve is linear in the range 2.0×10⁻⁷—5.0×10⁻¹² M with a detection limit of 2.0×10⁻¹² M (ca. 0.7 pg/ml). The method maybe applied direct determination of the drug in pharmaceutical and biological samples. For a concentration of 5.0×10⁻⁸ M a recovery value of 99.89% is obtained.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 57 (2), 117-121, 2009

  公益社団法人 日本薬学会

キーワード

• fast Fourier transformation
• square-wave voltammetry
• gold ultramicroelectrode
• flow-injection
• ketotifen

詳細情報

• CRID: 1390282679147775360
• NII論文ID: 110007042067
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.57.117
• ISSN: 13475223
• ISSN: 00092363
• NDL書誌ID: 9775022
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9775022
• Web Site: http://www.jstage.jst.go.jp/article/cpb/57/2/57_2_117/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009176380
• 本文言語コード: en
• データソース種別:
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  • NDL
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• 抄録ライセンスフラグ: 使用不可