Effects of Pro-Arg, a Novel Dipeptide Derived from Protamine Hydrolysate on H2O2-Induced Oxidative Stress in Human Diploid Fibroblasts
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- Wang Yonggang
- Shanghai Institute of Materia Medica, Chinese Academy of Sciences Global R&D Center of Tianjin Tiens Group
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- Zhu Fengrong
- Global R&D Center of Tianjin Tiens Group
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- Chen Jie
- Shanghai Institute of Materia Medica, Chinese Academy of Sciences
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- Han Fusen
- Global R&D Center of Tianjin Tiens Group
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- Wang Heyao
- Shanghai Institute of Materia Medica, Chinese Academy of Sciences
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Abstract
Oxidative stress plays an important role in tissue damage associated with many different chronic degenerative diseases, such as cancer and cardiovascular disorders. In our previous study, a novel dipeptide Pro-Arg derived from protamine hydrolysates was found to possess significant antioxidative activity in vitro. In this study, we compared the antioxidant capabilities of purified and synthesized dipeptide (Pro-Arg) and found both of them had very strong hydroxyl-radical scavenging activity in vitro, even at a very low concentration. The protective effect of the dipeptide against oxidative stress was evaluated using H2O2-induced oxidative stress of human diploid fibroblasts MRC-5 cell model. Our results showed the dipeptide attenuated H2O2-induced cellular oxidative damage, and normalized S phase arrest of MRC-5 cells exposed to H2O2. Our findings demonstrate that Pro-Arg can protect against oxidative stress/damage and H2O2-induced human diploid fibroblasts cell death.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 32 (3), 389-393, 2009
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204625147776
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- NII Article ID
- 110007122729
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 10166281
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed