遊離歯肉移植術における上皮の角化状態の免疫組織学的観察  [in Japanese] Immunohistological Observations of Epithelial Keratinization after Free Gingival Graft Procedures  [in Japanese]

In this study, immunohistological observations were performed using two kinds of epithelial markers to determine the maintainability of the original characteristics after free gingival graft procedures. Matertais and Methods: One hundred and twenty male Wistar rats were used. After the free gingival graft procedures, the animals were sacrificed 1, 2, 4, and 6 weeks postoperatively. Conventional histological sections were made. Involucrin and Cytokeratin 10 were used for the immunohistochemical analysis. Antibody positive rate within the epithelial layer was calculated. Results: Stainability against Involucrin and Cytokeratin 10 did not change until 6 weeks postoperatively, and the grafted site showed the similar stainability as in the palate. The percentage of positive cell rate after 6 weeks postoperatively was close to that of the palate. Discussion: The histological as well as immunohistochemical characteristics of palatal keratinization were maintained after the free gingival procedures.